Breeding Considerations for Rats -------------------------------- Females rats should not be bred before 65 days of age. They are continuously polyestrous, which means that they come into heat at fairly regular intervals (every 4-5 days) throughout the entire year unless they are bred. The period during which the female is receptive to the male and allows breeding is about 12 hours and usually occurs at night. Female rats can come back into heat 48 hours after giving birth to a litter. This is called a postpartum estrus. This period of receptivity is not used when breeding rats because the breeding male is removed from the enclosure just before the female delivers her litter because of the high probability of injury to the new pups by the male. After mating, a white, waxy substance, called a copulatory plug, is visible within the female's vulva for 12-24 hours. It is not uncommon to find these plugs within the enclosure after they have been discharged. Pregnancy lasts an average of 3 weeks. Litter sizes average 6-12 pups, though it is not unusual for a female's first litter to be smaller in number. Litter sizes decrease as breeding females age. Female rats should not be disturbed for the first few days after delivery because stressed females may destroy their pups. Excessive handling, loud noises, and even insufficient nesting material have all been implicated with this destructive behavior. Pups are usually weaned at about 3 weeks of age. The female resumes her breeding cycle 2-5 days after her pups have been weaned.